Category Archives: Thin Layer Chromatography

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Welcome to the first blog dedicated to Thin Layer Chromatography!

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Thin Layer Chromatography advocated in Forensic Magazine

Oliver Grundmann, Ph.D. and Ian Tebbett, Ph.D.   have published a piece in the February/March issue of Forensic Magazine in which they advocate the use of Thin Layer Chromatography. Here’s an excerpt:

Forensic Magazine“Other methods for the detection of mycotoxins include rapid screening by thin layer chromatography (TLC) as well as radio immunoassay (RIA) or enzyme-linked immunosorbent assays (ELISA) in serum, urine, gastric, and stool samples. A recently introduced ELISA for amanitine has been evaluated for its use in determining Amanita phalloides poisonings in urine, serum, and fecal samples. The ELISA-based approach yielded sufficient sensitivity and specificity compared to LC/MS and RIA methods in urine samples if analysis occurred within the first 36 hours after ingestion. Since ELISA kits do not require complex extraction procedures or the use of radioactivity, this might provide a time- and cost-effective alternative.

As low-tech as TLC might appear to be, it has been frequently used for qualitative identification of a variety of mycotoxins in biological fluids. Orellanine, a toxin from the mushroom Cortinarius orellanus or Fool’s webcap, has been identified by TLC in serum and urine after photodecomposition to orelline.”

This is Really HOT!

Did you know that it was Thin Layer Chromatography that first allowed us to measure exactly how “hot” spicy food is?hot pepper

The presence of other compounds related to capsaicin in Capsicum extracts was detected by Kosuge et al. (1961) and Friedrich and Rangoonwala (1965) using paper and thin-layer chromatography methods respectively. Kosuge named the new compound in his sample dihydrocapsaicin, which was observed present in a ratio of 3 : 7 with capsaicin. Other investigations by Todd and Perun (1961), Morrison (1967), Hollo eta!. (1969) and Hartman (1970) also indicated the occur- rence of one or more compounds related to capsaicin in extracts of products.

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From another great Blog: PRACTICAL 4: SEPARATION OF LIPIDS VIA THIN LAYER CHROMATOGRAPHY

We love to see Chromatography written about all over the place. So whenever something good is brought to our attention, we want to pass it along to you.

This is from Biochemistry Practicals:

In this practical the lipids present in egg yolk were separated through absorption chromatography using a TLC plate. The concept is similar to paper chromatography, a plate containing a thin hydrophilic layer called a matrix is used, and a non polar solvent is allowed to move up the plate through capillary action. Thus more polar lipids will move more slowly than non polar lipids.

Click Here to read the full post.

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