Category Archives: Thin Layer Chromatography

Getting a Grip on Your Chromatography: “Why Me?” – Part I

Have you ever pulled your TLC plate out of the developing tank, only to realize that your Rfs are out of whack? Or the analyte you were expecting to see is missing or still overlapping another component? Time is wasted. Emotions flare. Did you forget something? Are the plates any good? Did you pick the wrong profession?

Many factors affect your results in chromatography. They include:

  • layer thickness
  • particle size and pore size of adsorbent
  • activation of adsorbent
  • mobile phase (quantity and freshness)
  • equilibrium inside the developing chamber
  • sample (size and application technique)
  • accurate Rf measurement

All of these controllable variables are important. To save time, you may be tempted to focus on the most influential and leave the others alone. However, some applications are more sensitive to slight variations. It’s better to invest time in the beginning to make sure every variable is just right. You’ll save time and energy—and will rest assured you’re indeed in the right profession. 

That’s why we’re publishing a series of articles to explain these contributing factors in detail. (We know how much our analytical audience loves details!) Our goal is to broaden your overall knowledge in chromatography and help you achieve more consistent results.

Stay tuned…and happy chromatographing!

New Chromatography website launched

Only U.S.- based Thin Layer Chromatography Plate manufacturer launches new site

NEWARK, DE – iChromatography/Analtech, the only U.S.-based manufacturer of Thin Layer Chromatography Plates, started 2015 with the launch of a new website.

The new site – – is designed to help visitors find their chromatography product faster and easier as well as discover new developments in the field of separation science.Screen shot 2015 01 05 at 1.38.17 PM resized 600

“Since we started more than 50 years ago, we’ve always been committed to providing not only great products, but the best customer service and information possible to our current and future customers,” said Steven C. Miles, General Manager for iChromatography/Analtech. 

The New site is designed by Custom House and features a state-of-the-art online store with more than 500 varieties of Thin Layer Chromatography Plates, HPLC columns, SPE Cartridges, automation devices, and more.

“We’re proud of the high quality chromatography products and technical assistance we’ve offered to customers in more than 70 countries over the last 54 years, and we’re looking forward to serving more customers in more countries for decades to come,” said Miles.


Some Applications of Thin Layer Chromatography 薄层层析法的一些应用



1. 样品的提纯:样品的提纯可以通过薄层层析法来完成。直接比较可在随机样品和标准或真实样品间进行;如果样品经检测不纯,那么一定有额外斑点存在,而这个很容易被发现。

2. 混合物的识别:薄层层析法可以被应用于天然产品的提纯、隔离和鉴定,其中包括挥发油、精油、固定油、蜡、萜烯、生物碱、苷类、类固醇,等等。

3. 反应监测:薄层层析法可以对反应混合物进行检测从而判断其反应是否完全。这个方法也可用于检查其他分离过程或提纯进程,比如蒸馏、分子蒸馏等等。

4. 生物化学分析:薄层层析法对于生物化学代谢物以及体液、血浆、血清和尿液构成物的隔离或分离检测特别有效。

5. 在化学中的应用:薄层层析法在相似成分的分离和识别实验中的应用等化学领域中越来越普及。同时,该方法也应用于无机化学中阴阳离子的鉴别。

6. 在医药学领域的应用:不少药典已经采用薄层层析法来检测化学药物的纯度。

7. 像催眠药、镇定剂、抗惊厥剂、抗组胺剂、镇痛剂、局部麻醉剂、类固醇等药物都可以用薄层层析法有效检测。

8. 薄层层析法另一个重要应用是对多成分药物配方的分离。

9. 食物和化妆品领域的应用薄层层析法也用于颜色、防腐剂、甜味剂以及各种化妆产品要素的分离和鉴别。

Advantages of Thin Layer Chromatography 薄层层析法的优点

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4. 薄层层析法样本的检测极限大约要比纸层析法低一个小数位,并且,分析所需的样本数目较少。

5. 在纸层析检验中,纤维素十分容易被破坏,因而腐蚀剂喷雾不能被用于鉴定实验,但对于薄层层析法而言,则不存在这一难题。

6. 可用吸附剂资源丰富,因而薄层层析法被广泛用于吸附、分离以及离子交换层析。

7. 相比于纸层析法,薄层层析法的个体样板不容易扩散,因而检测敏感度更强。

8. 被分离的成分可以通过金属粉末涂料的刮擦来达到恢复,同时,点状或区域的量化分离也可以得到实现。

9. 无机吸附背景下不透明,因而我们可以在检测鉴定中通过紫外光来识别每一种成分。

10. 薄层层析法可以在较厚的层析吸附剂的作用下用于预备性分离。

Click Here for more

Applications and Advantages of Thin Layer Chromatography

We are thrilled to find web sites, blogs, videos, and posts centered around chromatography, and we enjoy sharing these finds!describe the image

Nitin Vasava is a pharmacist who created the blog pharmastuff4u.

Nitin has posted a couple of very useful lists, here’s excerpts:

TLC Application | Some Applications of Thin Layer Chromatography


  1. Purity of  any sample : Purity of sample can be carried out with TLC. Direct comparison  is done between the sample and the standard or authentic sample; if any impurity is detected, then it shows extra spots and this can be detected easily.
  2. Identification of compounds: Thin layer chromatography can be employed in purification, isolation and identification  of natural products like volatile oil or essential oil, fixed oil, waxes, terpenes, alkaloids, glycosides, steriods etc.
  3. Examination of reactions: Reaction mixture can be examined by Thin layer chromatography to access whether the reaction is complete or not. This method is also used in checking other separational processes and purification processes like distillation, molecular distillation etc.

Click Here for the complete post.

Advantages of Thin Layer Chromatography TLC

Advantages of Thin layer chromatography
Advantages of Thin layer Chromatography (TLC)


  1. Less equipment is required.
  2. Very little time for separation is required.
  3. It is more sensitive.
  4. The lower detection limit of analytical sample in TLC is approximately one decimal lower than that is paper chromatography and very small quantities of sample is sufficient for analysis.

Click Here for the complete list.

Collection of Thin Layer Chromatography Videos

There are a number of videos available that demonstrate thin layer chromatography – we figured it might be helpful to put some of these here as an easy reference.

NOTE: we were not involved with the production of any of these videos, we want to make sure you give credit where credit is due – so we’ve included any information we can from the individuals and organizations responsible for these productions. Also, these videos are offered in no particular order.

Please feel free to send along any videos to add to the collection.

1. O-Chem Thin Layer Chromatography with Dr. John Davison at Irvine Valley College, in Irvine, Ca. (Part 6 of 7) of the IVC Chemistry Lab Safety Series. 

2. Unknown producer


 3. Thin Layer Chromatography 
Demonstrated by Mark Niemczyk, PhD

4. Spartan calculated molecule structures and Blender 3D rendered animation

5. Thin-layer Chromatography is used to separate the components of a drug mixture by polarity on a thin plate.

Thin Layer Chromatography helps in discovering new fat over lunch

We are firm believers in inspiration and solutions coming to us at any place and any time – so we were happy to see this story:

Scientists discover new fat over lunch

Uncovering new fats, or lipids, with links to diseases in the human eye is as easy as taking a lunch break, according to UOW chemists from the ARC Centre of Excellence for Free Radical Chemistry and Biotechnology.Dr. Blanksby

The research, conducted by UOW Associate Professor Stephen Blanksby and his PhD student Shane Ellis, used ozone naturally present in laboratory air to find previously unknown lipids in the human lens. Their work was featured as a hot article in a recent edition of the scientific journal, Analyst.

“My lunch breaks are never unproductive,” according to Shane.


Using a combination of two methods called desorption electrospray ionisation mass spectrometry (DESI) and thin layer chromatography (TLC) that the group combined specifically for these experiments, lipids can be characterised in one simple analysis, a significant improvement on older methods.

Click Here to read the complete article.

We tip our hats to Professor Blanksby and Mr. Ellis.