Category Archives: Export

Celebrating the birth of the father of Chromatography

Mikhail Semyonovich Tsvet was born on this day in 1872.Tsvet

Here’s an excerpt from Discoveries in Medicine about the significance of Tsvet’s work:

The first chromatograph was invented by Russian botanist Mikhail Semenovich Tsvett (1872-1919). While working in Poland, Tsvett was looking for a method of separating a mixture of plant pigments (tints) which are chemically very similar to each other. To isolate different types of chlorophyll, he trickled a mixture of dissolved pigments through a glass tube packed with calcium carbonate powder. As the solution washed downward, each pigment stuck to the powder with a different degree of strength, creating a series of colored bands. Each band of color represented a different substance. Tsvett referred to the colored bands as a chromatogram. He also suggested that the technique (now called adsorption chromatography) could be used to separate colorless substances.

Although Tsvett published a report of his work in the early 1900s, chemists paid very little attention to it. There were a few reasons for ignoring the work. First, the report was written in Russian, which few Western chemists of the time read. Second, the technique may have seemed too simple to chemists who were used to relying on lengthy extraction, crystallization, or distillation processes to separate mixtures. Within a few years, Tsvett’s technique was rediscovered. The rediscovery was by the German organic chemist Richard Martin Willstatter (1872-1942), who was also studying chlorophyll. By introducing chromatography to Western European scientists, Willstatter helped establish one of the most versatile analytical techniques known to chemistry.

Join us today as we honor the work of Mikhail Semyonovich Tsvet

Thin Layer Chromatography and Ophthalmology

This bit of insight comes from our friends at Medical Blog.

corneaA variety of genetic, metabolic, developmental, and idiopathic causes can result in congenital clouding of the cornea. A common reason for congenital clouding of the cornea is congenital glaucoma. Other major causes of corneal clouding include the following: Birth trauma Peters anomaly Dermoid tumors (limbal dermoids) Sclerocornea Congenital hereditary endothelial dystrophy (CHED) Mucopolysaccharidoses Infectious/inflammatory processes The following is a mnemonic for the causes of congenital clouding of the cornea: S – Sclerocornea T – Tears in the Descemet membrane secondary to birth trauma or congenital glaucoma U – Ulcers M – Metabolic P – Peters anomaly E – Edema (CHED) D – Dermoid Other rarer causes of congenital clouding of the cornea include the following: cornea plana, corneal keloids, oculoauriculovertebral (OAV) dysplasia (Goldenhar-Gorlin syndrome), congenital corneal ectasia, congenital hereditary stromal dystrophy, posterior polymorphous dystrophy, and Fryns syndrome.

A 4-month-old male infant had severe corneal opacity since birth. Examination revealed buphthalmos, increased IOP, and corneal opacity with neovascularization but not a dysmorphic face or hirsutism. The liver and spleen were impalpable. Hypotonia, poor head control, and absence of Moro and grasping reflexes were noted. He had no evidence of congenital infection (toxoplasmosis, other infections, rubella, cytomegalovirus infection, and herpes simplex [TORCH] study). Urine and plasma amino acid levels were normal. However, thin-layer chromatography showed excessive urinary excretion of heparan sulfate. Corneal transplantation was performed at 6 months of age. Histopathology of the corneal button showed homogeneous thickening of the Bowman layer and pinkish intracytoplasmic substances in the corneal stroma. The Alcian blue stain was positive, consistent with MPS of the cornea.

Click Here to read the full report.

Monty Python and Thin Layer Chromatography – the “I’m Not Dead Yet” chemical

Chemical & Engineering News just published a fascinating Antspiece about how ants let other ants know whether they are dead or not.

Of course, the whole piece starts off with a reference to Monty Python’s great “I’m not dead yet” scene from the Holy Grail. It’s good to see yet another connection between Monty Python and Chromatography!

Here’s an excerpt from the article:

By using thin-layer chromatography and gas chromatography to compare live, just-killed, and dead ants, the team showed that a subset of chemicals disappeared from the ants’ outer covering within one hour of death. From those extracts they identified two rapidly dissipating “do not discard” signaling chemicals—specific isomers of the natural products dolichodial and iridomyrmecin.

Click Here to read more and view the video.

Thin Layer Chromatography used in identifying Sixth Nucleotide

From our friends at GenomeWeb.com:

Two researchers from Rockefeller University have identified a new nucleotide…Synthesis of IMP

While evaluating 5-methylcytosine levels in two types of mouse brain cells, the team detected a nucleotide that they could not identify. When they looked more closely at this nucleotide using thin layer chromatography, high pressure liquid chromatography, mass spectrometry, and other approaches, the researchers discovered that they were dealing with 5-hydroxymethylcytosine, a form of methylated cytosine found stably in bacterial viruses.

Their subsequent experiments suggest the nucleotide is enriched in brain cells but apparently absent from several other cell types. Based on these findings, the researchers speculated that 5-hydroxymethylcytosine may contribute to epigenetic regulation, particularly in neurons. 

Click Here to read more from GenomeWeb.com

Thin Layer Chromatography used in DNA aging research

Excerpts from a paper posted on the U.S. National Library of Medicine and the National Institutes of Health web site DNA strandstitled:

TLC-based detection of methylated cytosine: application to aging epigenetics

5-Methylcytosine (m(5)C) has a plethora of functions and roles in various biological processes including human diseases and aging. A TLC-based fast and simple method for quantitative determination of total genomic levels of m(5)C in DNA is described, which can be applicable to aging research with respect to rapid and high throughput screening and comparison. Using this method, an example of the analysis of global alternations of m(5)C in serially passaged human skin fibroblasts is provided, which shows age-related global hypomethylation during cellular aging in vitro.

Click Here to access the paper.

CDC recommends Thin Layer Chromatography to test anti-malarial drugs

The U.S. Centers for Disease Control and Prevention advise that, “Counterfeit (fake) drugs are products deliberately made to resemble a brand name pharmaceutical. They may contain no active ingredients or contain ingredients inconsistent with the package description.”
Malaria treatment
For example, the CDC says, “In Cambodia in 1999, counterfeit antimalarial drugs were responsible for the deaths of at least 30 people. A recent survey in Southeast Asia showed that among 104 tablets presented as the antimalarial drug artesunate, 38% did not contain any artesunate.”

Users of pharmaceutical products (not only antimalarials) should take the following precautions:

  • Travelers should purchase in advance, in their home country, all the medicines they will need.
  • Travelers should record the drug’s generic and brand names as well as the name of the manufacturer; should they run out, they can look for the correct product.
  • Make sure that the drug is in its original packaging.
  • Inspect the packaging because many times poor quality printing indicates a counterfeited product.
  • Be suspicious of tablets that have a peculiar odor, taste or color, or that are extremely brittle.

The CDC recommends testing suspicious drugs.

“drug quality can be evaluated in the field by two simple, effective, and low-cost techniques: thin-layer chromatography (TLC) and colorimetry… The TLC technique consists of placing a spot of drug sample on a thin layer of silica attached to a plate of glass, aluminum, or plastic. The plate is then inserted into a vessel containing a solvent mixture. By capillary action, the solvent mixture creeps up the silica material and dissolves the sample. The drug sample consists of a mixture of drug and inactive ingredients. These compounds will have various affinities to the silica matrix and will migrate with the solvent at various speeds. This characteristic effectively separates out a mixture of compounds. After migration of the solvent is complete, individual components can be visualized by chemical treatment or ultraviolet (UV) absorbance. The distance that the components migrate is characteristic for each compound; therefore the active ingredient can be recognized by comparison with a known drug standard. The solvent can be modified to increase resolution between various components. This method is relatively inexpensive, specific, and sensitive. It is commonly used to assess drug quality.”

Click Here for more details from the CDC.

Click Here to find out more about Thin Layer Chromatography plates and accessories.

Separation of Lipids via Thin Layer Chromatography

This post comes to us from “Biochem write up

Intro:
The aim of this practical is to carry out TLC in which the substances we wish to separate are absorbed onto the thin layer. Lipids are determined by isolation and the ability to purify the substance. Due to the way the substances interact with the matrix in different ways we are able to seperate them. Substances which interact strongly with the matrix but not with the solvent will move very slowly and those soluble in the solvent will dissolve easily and be carried along the solvent.
thin layer chromatography phospholipids
Egg yolk contains trioleine, cholesterol palmitate and phosphatidylethonolamine, The most polar of the three elements is the second element. It hardly moves up the TLC because of the presence of several polar groups; phosphate groups, amine groups and several oxygens , and cholesterol palmitate being the most non polar.

 

Moving to a four day week!

It’s official!

As part of Analtech’s ongoing efforts to conserve energy and reduce our carbon footprint, we will start working four-day weeks in May!

This means a change in hours for us (10 hours a day for four days instead of 8 hours a day for five days) – but we don’t forsee any changes in the quality or timeliness of delivery for our customers.

What we do see is one less day a week for employees to commute to work and one less day of running heating/air conditioning.

The men and women here are pretty excited about the move – for us it means one more day a week to spend with our friends and family.

What about you? What is your company doing to conserve energy? 

Why the USDA uses Analtech TLC Plates

William Koskinen is a Soil Scientist with the U.S. Department of Agriculture. His current research interests include soil chemistry/biochemistry with an emphasis in determination and quantification of the mechanisms and factors controlling the degradation, movement, and activity of pesticides and their metabolites in soil; and use this knowledge to increase pesticide efficacy in agricultural production systems and decrease the risk of ground and surface water contamination.

William Koskinen has been performing Thin Layer Chromatography separations for about 30 years – and he uses Analtech Thin Layer Chromatography Plates.